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Overview
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Fusion genes are caused by chromosomal aberrations such as translocations, duplications, inversions or small interstitial deletions. At the transcript level, fusion genes may not only reflect underlying genomic rearrangements, but may also arise as a result of aberrant transcription or trans-splicing events. Fusion genes are a major cause of cancer, accounting for approximately 20% of human cancer incidence. However, the prevalence of fusion genes varies widely across cancers and many fusion genes are specific to certain cancer subtypes. Therefore, rapid and accurate identification of fusion genes can characterize and stratify a cancer diagnosis and inform subsequent treatment.


Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (RT-PCR) methods are primarily used for fusion gene diagnosis. Although highly sensitive, these methods typically test for the presence of only a single fusion gene, often resulting in a lengthy, iterative and costly diagnostic process. In addition, these methods are unable to identify novel fusion gene partners or resolve complex structural rearrangements.
Welcome to the river, estuary, and coastal restoration case studies '''RiverWiki'''. This site is funded by the '''Environment Agency''' (England) and supported by '''the RRC''' (UK).
'''This is an interactive source of information on river restoration, estuary restoration, coastal restoration and nature-based solution schemes from around Europe'''


With the avant-garde advent of RNA sequencing (RNA-seq) and Nanopore sequencing technologies, the landscape of fusion gene detection has witnessed a paradigmatic shift. These technologies elucidate full-length transcript sequencing with unprecedented read lengths, proffering profound insights into gene fusion dynamics.
Up to now, the database holds '''{{#ask:[[Category:Case study]]|format=count}}''' restoration case studies from '''31''' countries


Traditional Short-read RNA-Seq for Detection of Fusion Transcripts
==Map of case studies==
[https://www.cd-genomics.com/longseq/transcriptomics-with-long-read-sequencing.html RNA-Seq] has long been a traditional method for transcriptome research, capable of identifying fusion transcripts. The sensitivity and specificity of the method depends on the sequencing depth, read length and quality, as well as the bioinformatics methods and parameters used. However, traditional short read length sequencing faces several challenges:
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Fragmentation and assembly: Sequencing involves fragmenting cDNA libraries and reading them in short sequences (~50-100 bases). After sequencing, computational assembly is required to infer the complete transcript sequence. This fragmentation often leads to misassembly, especially in the case of detecting split-sequence (SAS) fusions.
<div style="float:right;clear:both;width:200%"><span style="float:right;">Left click to look around in the map, and use the wheel of your mouse to zoom in and out.</span></div>
Complex genomic regions: Short read lengths make it difficult to capture complex genomic rearrangements, repeat-rich regions, or full-length transcripts. Such limitations require complex computational analyses to infer full-length transcript sequences, leading to the potential omission of biologically significant variants.
To circumvent these challenges, bioinformaticians have developed two main strategies: a mapping-first approach, which identifies inconsistent reads indicative of genomic rearrangements; and an assembly-first approach, in which reads are assembled into longer transcript sequences to discover fusion transcripts.


Synthetic long read length (SLR) sequencing has been introduced as an alternative, aiming to combine the advantages of the thoroughness of long read length sequencing with the cost-effectiveness and accuracy of short read length sequencing. Here, short reads sharing the same barcode are compiled to construct longer reads.SLR-seq has been successfully used to identify large-scale isoform redistribution and several previously unknown fusion isoforms in benign colonic mucosa, primary colon cancer and metastatic colon cancer. While SLR provides deeper insights, it still relies on short reads as the basic assembly unit, limiting its efficacy to specific regions and capturing large numbers of repeats.
== Contents ==


Fusion Transcript SequencingTheoretical model of RNA-mediated gene fusions. (Dorney R et al., 2023)
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Long-read RNA-Seq: New Possibilities for Fusion Transcript Discovery
This tool is for sharing best practices and lessons learnt for policy makers, practitioners and researchers of river, estuary, and coastal restoration. The tool has information on water from source to sea.  
Advances in long-read sequencing technologies such as PacBio and Oxford Nanopore Technology (ONT) have enabled the generation of read lengths of tens of kilobases in length at relatively low cost, providing a more comprehensive view of transcripts. Although longer read length sequencing is more expensive than short read length sequencing, long read lengths can produce more accurate fusion predictions with the following advantages:


[https://www.cd-genomics.com/longseq/services.html Full-length sequencing]: the ability to span the full length of a transcript improves localization accuracy and promotes unambiguous identification of fusion transcripts.
=='''What you can do:'''==
- You can '''search''' the database to find case studies by using the different categories: country; monitoring or implementation costs and many more: [[Special:RunQuery/Case study query simple| click here to search for a case studies]]


Complex isoform detection: complex multi-exon isoforms, large transcripts, and complex fusion types (e.g., double-hop and bridging fusions) can be resolved without computational inference.
- You can also '''search''' the database to find case studies by topic e.g. natural flood risk management: [https://restorerivers.eu/wiki/index.php?title=Special%3ARunQuery/Case_study_query_simple_with_map&wpRunQuery=true&Case_study_query_simple%5BThemes%5D=Flood%20risk%20management&Case_study_query_simple%5BUnit%5D=km&Case_study_query_simple%5BResult%20type%5D=Map Click here to search for all the natural flood risk management case studies]]


Oxford Nanopore's MinION system stands out for its real-time data generation capabilities. Using this system, assays have been developed that can detect oncogenic gene fusions in a very short period of time. By modifying the anchored multiplex PCR method for library creation, fusions such as BCR-ABL1 can be identified within minutes of sequencing initiation.
- Please also '''add''' your own river restoration scheme to the database: {{Create case study link|Text=click here to create a new case study}}.


using the concepts written previously, rewrite this article with a high degree of complexity and specificity:
- Provide us with your '''feedback''': please add to the discussion pages.


Workflow for Long-Read RNA-Seq Detection of Fusion Transcripts
'''HAVE YOUR SAY''', we are happy to receive any suggestions for improvements to the site [[RESTORE Contacts|please contact us]].
(1) Fusion Transcript Enrichment


To ensure accurate detection of fusion transcripts, the first step is enrichment, using two main techniques:
''The RiverWiki has been developed by the RESTORE partnership for sharing knowledge and promoting best practice on river restoration. The RESTORE partnership is made possible with the contribution of the LIFE+ financial instrument of the European Community.
[http://www.restorerivers.eu/About/RESTOREProject/tabid/2607/Default.aspx Read more on the RESTORE partnership.]''


Sequence-specific RT-PCR:
== Countries ==
The following countries are members of the RESTORE partnership.  Click any of the links below to view information about that country.


When dealing with a known complete transcript, a sequence-specific RT-PCR approach is adopted. This is a highly targeted amplification technique tailored to ensure the detection and amplification of the exact fusion transcript under scrutiny. Its precision stems from its ability to focus solely on the fusion transcript of known sequence composition.
{{#ask:[[Category:RESTORE country]]|limit=100}}


Semi-specific RT-PCR:
The following European countries are not members of the RESTORE partnership, but can also be clicked to view information about the country.


In instances where the knowledge encompasses only one end of the fusion transcript, semi-specific RT-PCR becomes the method of choice. This technique amplifies transcripts by leveraging known fragment sequences, thus presenting a pathway to unearth previously undetected fusion events.
{{#ask:[[Category:European country]] [[Not a RESTORE country::Yes]]|limit=100}}


(2) Sequencing
== Search ==
* [[Special:RunQuery/Case study query simple| Search for case studies using a basic search form]]
* [[Special:RunQuery/Case study query comprehensive| Search for case studies using an advanced search form]]


After the enrichment phase, the next procedural step is the meticulous preparation of the library. At this juncture, the PCR-cDNA Sequencing Kit emerges as a pivotal tool. It presents a streamlined mechanism to efficiently transmute enriched RNA samples into sequencing-ready libraries, ensuring the fidelity of the transcript representation.
== Create a case study ==
{{Create case study link}}


(3) MinION and Flongle Flow Cells: determining the size and depth of sequencing
== Contacts ==
'''Do you have ideas of things that could be improved?''' [[RESTORE Contacts|please contact us]].


MinION:
Please add your thoughts to the [[Talk:Main Page|discussion page]] or if you have any questions about the RESTORE case studies wiki or feedback, you can find a list of contacts on the [[RESTORE Contacts|contacts page]].


The MinION's compact footprint belies its capabilities. It's a quintessential device for real-time sequencing applications, boasting versatility that allows its deployment in standard laboratory conditions or even in more challenging remote research environments.
== Other resources ==
* [http://www.gwp.org/en/ToolBox/ The IWRM ToolBox] is a free and open database with a library of background papers, policy briefs, technical briefs and perspective papers as well as huge sections of case studies and references in each tool.
* [http://www.iwawaterwiki.org The IWA WaterWiki] is an online resource for all areas of water, wastewater and environmental science and management. It has a [http://www.iwawaterwiki.org/xwiki/bin/view/Main/Tags?do=viewTag&tag=Rivers section specifically dedicated to rivers] as well.
* The REFORM wiki [http://wiki.reformrivers.eu/index.php/Main_Page a wiki site] disseminates scientific knowledge about river restoration.
* [http://wateractionhub.org/ The Water Action Hub] is an online platform designed to assist stakeholders to efficiently identify potential collaborators and engage with them in water-related collective action to improve water management in regions of critical strategic interest.


GridION:
The RiverWiki was developed by the '''RESTORE EU LIFE+ partnership''', which was made possible with the contribution of the LIFE+ financial instrument of the European Community.  
 
[[File:Life.png|frameless|right|top|upright]]
Engineered with multifaceted projects in mind, the GridION houses up to five individually addressable flow-through tanks. This intricate design strikes a harmonious chord between scalability-catering to expansive sequencing needs-and the granularity of a detailed molecular analysis.
 
(4) Sequencing Equipment: Flexibility in the sequencing process can be increased by choosing equipment
 
MinION:
 
The MinION's compact footprint belies its capabilities. It's a quintessential device for real-time sequencing applications, boasting versatility that allows its deployment in standard laboratory conditions or even in more challenging remote research environments.
 
GridION:
 
Engineered with multifaceted projects in mind, the GridION houses up to five individually addressable flow-through tanks. This intricate design strikes a harmonious chord between scalability – catering to expansive sequencing needs – and the granularity of a detailed molecular analysis.
 
(5) Data Analysis
 
Post-sequencing, the generated data necessitates rigorous computational and statistical scrutiny. This phase involves the application of robust bioinformatics tools and algorithms to ascertain the veracity of detected fusion transcripts, quantifying their abundance, and contextualizing their potential biological implications.
 
Fusion Transcript SequencingMultistep anchored multiplex PCR (AMP)-based library preparation for MinION sequencing and turnaround time. (Jeck W R et al., 2019)
 
Bioinformatic Approaches for Fusion Detection in Long-read Data
LongGF: A Pioneering Approach
 
One of the first tools tailored for long-read fusion detection was LongGF. Utilizing Minimap2 for genome alignment, it offers a prioritized list of potential gene fusions. A unique feature of LongGF is its ability to filter out overlapping genes and alignments, clustering reads for a more concise fusion detection. However, its reliance on predefined genomic coordinates can be a double-edged sword. While it provides specificity, it may miss out on detecting fusions involving uncharacterized genes or exons. Moreover, the sequence similarity between homologous genes might pose challenges in determining the origin of fusion partners.
 
Genion: Stringent Fusion Filtering
 
To address the potential pitfalls of false positives, Genion emerged with more stringent thresholds. Using desalt for genome alignment, this tool applies filters not to individual reads but to entire read clusters. Such an approach offers powerful filtering, presenting cleaner candidates for analysis. Genion's strength lies in its ability to discern between genuine fusion events and mapping errors or genomic variants. However, its rigorous filtering might sometimes be over-cautious, leading to potential overlooks of valid fusion events.
 
AERON: Translating Reads to Transcriptomes
 
A standout tool, AERON, opted for a different alignment strategy. Using GraphAligner, AERON aligns reads directly to a reference transcriptome, bypassing the genome. This approach allows for the quantification of transcripts, translating read counts into Transcripts Per Million values. While innovative, aligning to the transcriptome introduces challenges, especially when dealing with highly similar short-length transcripts.
 
JAFFAL: Dual Alignment for Precision
 
Building upon the foundation of its predecessor, the short-read fusion caller JAFFA, JAFFAL employs a two-pronged alignment strategy. Using Minimap2, reads are first aligned to a reference transcriptome, followed by a secondary alignment to a reference genome for reads indicating potential fusions. This dual-alignment strategy not only minimizes false positives but also streamlines computational demands, given that only a subset of the reads undergo genome alignment.
 
References
 
Dorney, Ryley, et al. "Recent advances in cancer fusion transcript detection." Briefings in Bioinformatics 24.1 (2023): bbac519.
Jeck, William R., et al. "A nanopore sequencing–based assay for rapid detection of gene fusions." The Journal of Molecular Diagnostics 21.1 (2019): 58-69.

Latest revision as of 10:21, 6 January 2025


Welcome to the river, estuary, and coastal restoration case studies RiverWiki. This site is funded by the Environment Agency (England) and supported by the RRC (UK). This is an interactive source of information on river restoration, estuary restoration, coastal restoration and nature-based solution schemes from around Europe

Up to now, the database holds 1483 restoration case studies from 31 countries

Map of case studies

Loading map...
Left click to look around in the map, and use the wheel of your mouse to zoom in and out.

Contents

Latest updated case studiesModification date"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.Country
Ullswater Catchment Restoration10 December 2024 14:47:34England
Greatham Marsh Restoration10 December 2024 14:29:09England
Rye Harbour Farm Regulated Tidal Exchange10 December 2024 10:45:19England
Swindale Beck Restoration10 December 2024 10:43:38England
Washwalk Wetland10 December 2024 10:41:55England
more..

This tool is for sharing best practices and lessons learnt for policy makers, practitioners and researchers of river, estuary, and coastal restoration. The tool has information on water from source to sea.

What you can do:

- You can search the database to find case studies by using the different categories: country; monitoring or implementation costs and many more: click here to search for a case studies

- You can also search the database to find case studies by topic e.g. natural flood risk management: Click here to search for all the natural flood risk management case studies]

- Please also add your own river restoration scheme to the database: click here to create a new case study.

- Provide us with your feedback: please add to the discussion pages.

HAVE YOUR SAY, we are happy to receive any suggestions for improvements to the site please contact us.

The RiverWiki has been developed by the RESTORE partnership for sharing knowledge and promoting best practice on river restoration. The RESTORE partnership is made possible with the contribution of the LIFE+ financial instrument of the European Community. Read more on the RESTORE partnership.

Countries

The following countries are members of the RESTORE partnership. Click any of the links below to view information about that country.

Austria, Belgium, Bulgaria, Denmark, England, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Netherlands, Northern Ireland, Norway, Poland, Portugal, Romania, Scotland, Slovenia, Spain, Sweden, Wales

The following European countries are not members of the RESTORE partnership, but can also be clicked to view information about the country.

Albania, Andorra, Armenia, Azerbaijan, Belarus, Bosnia and Herzegovina, Croatia, Cyprus, Czech Republic, Georgia, Iceland, Latvia, Liechtenstein, Lithuania, Luxembourg, Macedonia, Malta, Moldova, Monaco, Montenegro, Russia, San Marino, Serbia, Slovakia, Switzerland, Turkey, Ukraine, Vatican City

Search

Create a case study

Click here to create a new case study

Contacts

Do you have ideas of things that could be improved? please contact us.

Please add your thoughts to the discussion page or if you have any questions about the RESTORE case studies wiki or feedback, you can find a list of contacts on the contacts page.

Other resources

  • The IWRM ToolBox is a free and open database with a library of background papers, policy briefs, technical briefs and perspective papers as well as huge sections of case studies and references in each tool.
  • The IWA WaterWiki is an online resource for all areas of water, wastewater and environmental science and management. It has a section specifically dedicated to rivers as well.
  • The REFORM wiki a wiki site disseminates scientific knowledge about river restoration.
  • The Water Action Hub is an online platform designed to assist stakeholders to efficiently identify potential collaborators and engage with them in water-related collective action to improve water management in regions of critical strategic interest.

The RiverWiki was developed by the RESTORE EU LIFE+ partnership, which was made possible with the contribution of the LIFE+ financial instrument of the European Community.

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